Download and read online Manipulating the Mouse Embryo in PDF and EPUB This fourth edition of "The Mouse Manual¨is once again the definitive reference source on mouse development, transgenesis techniques, and molecular biology. Its preeminent authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy have reorganized and updated this edition to include new information and protocols on: · generation of induced pluripotent stem cells · RNA microinjections · lentiviral microinjections and infection · assisted reproduction techniques for sperm and embryo cryopreservation · isolation, generation, and transplantation of spermatogonial stem cell lines · in utero electroporation of gene constructs into postimplantation embryos · vibratome sectioning of live and fixed tissues for imaging thick tissue sections · whole-mount fluorescent staining methods for three-dimensional visualization Recombinant DNA techniques and methods for studies of mouse embryonic development have been updated from previous editions, as has the wealth of information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups. The first edition of this classic work appeared more than 20 years ago, with authors that included Brigid Hogan, Rosa Beddington, Frank Costantini, and Elizabeth Lacy. The field's technological sophistication has grown exponentially but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice.
Download and read online Manipulating the Mouse Embryo in PDF and EPUB A comprehensive source of technical guidance for experienced investigators and essential resource for newcomers to mammalian genetics and embryology. This edition (1st ed., 1986) is revised and expanded to incorporate improvements to existing methods as well as new technologies. It contains new sect
Download and read online A Laboratory Guide to the Mammalian Embryo in PDF and EPUB Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.
Download and read online Mouse Phenotypes in PDF and EPUB The generation of mutant mice raises many questions about the best means of phenotypic analysis, breeding, and maintenance. The answers are now available from two experts with a wealth of detailed knowledge never previously assembled in one volume. Informal and highly practical, this handbook provides stepâ€“byâ€“step methods for troubleshooting experiments, from the basics of gene targeting through the analysis of postnatal effects.
Download and read online Gene Knockout Protocols in PDF and EPUB As the major task of sequencing the human genome nears completion and the full complement of human genes are catalogued, the task of understanding the normal biological functions of genes and how their alteration leads to diseased states becomes more imperative. In Gene Knockout Protocols, highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for genetic phenotype analysis. These proven methods contain step-by-step instructions, as well as notes on pitfalls to avoid, and emphasize techniques that are relevant to researchers carrying out gene targeting work. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. Issues such as the use of existing mouse mutation resources and the influence of genetic background and epigenetic effects upon phenotype are also covered. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.
Download and read online Molecular Cloning in PDF and EPUB The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques. The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.
Download and read online Mouse Genetics and Transgenics in PDF and EPUB This unique book integrates knowledge from a wide range of expertise, specifically applied to the mouse and addressed at a wide audience fro m those new to the field to experts who want an update on the state of the art. Mouse Genetics and Transgenics: A Practical Approach covers all aspects of using the mouse as a genetic model organism: care and h usbandry; archiving stocks as frozen embryos or sperm; making new muta tions by chemical mutagenesis; transgenesis; gene targeting; mapping m utations and polygenic traits by cytogenetic, genetic, and physical me ans; and disseminating and researching information via the Internet.
Download and read online Molecular Embryology in PDF and EPUB In Molecular Embryology, expert investigators provide a comprehensive guide to the cutting-edge methods used today across the dramatically growing field of vertebrate molecular embryology. These powerful techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The major techniques of experimental molecular biology and the particular advantages of each different species are emphasized. Detailed, easy-to-follow protocols, together with relevant background information and helpful tips, optimize the methods for success. Molecular Embryology brings together in one volume all the major techniques and common experimental species needed to study the mechanisms of biological development in vertebrates. Bound to become a standard reference in this field, the book makes it possible for experienced and novice researchers alike to move between embryos of diverse vertebrate classes as their project progresses, ensuring their ability to utilize the experimental advantages of different systems to address specific developmental questions.
Download and read online Mouse Development in PDF and EPUB This book represents a classic compilation of current knowledge about mouse development and its correlates to research in cell biology, molecular biology, genetics, and neuroscience. Emphasis is placed on the research strategy, experimental design, and critical analysis of the data, disguishing this from other books that only focus on protocols for mouse developmental research. Selected chapters are indexed to electronic databases such as GeneBank, GenBank, Electronic Mouse Atlas, and Transgenic/Knockout, further increasing the utility of this book as a reference. *Broad-based overview of mouse development from fundamental to specialist levels *Extensive coverage of a wide range of developmental mutations of the mouse *Excellent benchmark illustrations of brain, craniofacial, gut and heart development *In-depth experiment-based assessment of concepts in mammalian development *Focus on models of specific relevance to human development *Comprehensive reference to key literature and electronic databases related to mouse development *High-quality full-color production
Download and read online The Atlas of Mouse Development in PDF and EPUB Not since the early 1970s has there been an attempt to describe and illustrate the anatomy of the developing mouse embryo. More than ever such material is needed by biologists as they begin to unravel the molecular mechanisms underlying development and differentiation. After more than ten years of painstaking work, Matt Kaufman has completed The Atlas of Mouse Development--the definitive account of mouse embryology and development. For all those researching or studying mammalian development, The Atlas of Mouse Development will be the standard reference work for many years to come. Provides a comprehensive sequential account of the development of the mouse from pre-implantation to term Contains clear and concise descriptions of the anatomical features relevant to each stage of development Large format for easy use Contains explanatory notes and legends, and more than 180 meticulously labeled plates, 1,300 photographs of individual histological sections, and 200 electron micrographs, illustrating: Intermittent serial histological sections through embryos throughout embryogenesis and organogenesis Differentiation of specific organs and organ systems, including the spinal cord, eyes, gonads, kidneys, lungs and skeletal system External appearance of intact embryos throughout development
Download and read online Transgenic Mouse in PDF and EPUB A collection of readily reproducible methods for working with mice, and particularly for generating mouse models that will enable you to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented. Use effective methods for conditional and inducible gene expression Review mouse models for a wide range of genetic aberrations associated with human disease.
Download and read online Transgenic Animal Technology in PDF and EPUB Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Fully revised with updates to reflect state-of-the-art technology and associated changes to date
Download and read online The Mouse in Biomedical Research in PDF and EPUB Normative Biology, Husbandry, and Models, the third volume in the four volume set, The Mouse in Biomedical Research, encompasses 23 chapters whose contents provide a broad overview on the laboratory mouse’s normative biology, husbandry, and its use as a model in biomedical research. This consists of chapters on behavior, physiology, reproductive physiology, anatomy, endocrinology, hematology, and clinical chemistry. Other chapters cover management, as well as nutrition, gnotobiotics and disease surveillance. There are also individual chapters describing the mouse as a model for the study of aging, eye research, neurodegenerative diseases, convulsive disorders, diabetes, and cardiovascular and skin diseases. Chapters on imaging techniques and the use of the mouse in assays of biological products are also included.
Download and read online Mouse Molecular Embryology in PDF and EPUB In Mouse Molecular Embryology: Methods and Protocols, expert researchers in the field detail many of the protocols used to study mouse embryology. These include protocols and techniques that are "close to the embryo": such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA. With additional chapters on fluorescence imaging, lineage tracing, and genetic ablation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mouse Molecular Embryology: Methods and Protocols seeks to aid scientist in the further study of mouse embryo and its relation to other aspects of biological research.
Download and read online Human Preimplantation Embryo Selection in PDF and EPUB The most profound dilemma in assisted reproduction to date is the inability to recognize potentially viable embryos before their replacement into the reproductive tract. Application of increasingly advanced new technology has allowed the field of embryo evaluation to evolve rapidly and dramatically over the past five years. Human Preimplantation Embryo Selection assembles the leaders in the field of assisted reproduction to discuss their visions of dealing with the topic of embryo selection in the light of the limitations involving the fragility of the early human embryo. The purpose of this book is to shed light on the ever-expanding techniques that are being employed and evaluated with regard to embryo selection. A comprehensive overview may guide the ART practitioner in choice of techniques and evaluation of benefits and drawbacks. This book will provide an up-to-date overview and reference source: an invaluable tool for assisted reproductive practice worldwide.