Download and read online Imaging in Developmental Biology in PDF and EPUB New imaging technologies have revolutionized the study of developmental biology. Where researchers once struggled to connect events at static timepoints, imaging tools now offer the ability to visualize the dynamic form and function of molecules, cells, tissues, and whole embryos throughout the entire developmental process. Imaging in Developmental Biology: A Laboratory Manual, a new volume in Cold Spring Harbor Laboratory Press' Imaging series, presents a comprehensive set of essential visualization methods. The manual features primers on live imaging of a variety of standard model organisms including C. elegans, Drosophila, zebrafish, Xenopus, avian species, and mouse. Further techniques are organized by the level of visualization they provide, from cells to tissues and organs to whole embryos. Methods range from the basics of labeling cells to cutting-edge protocols for high-speed imaging, optical projection tomography, and digital scanned laser light-sheet fluorescence. Imaging has become a required methodology for developmental biologists, and Imaging in Developmental Biology: A Laboratory Manual provides the detailed explanations and instructions for mastering these necessary techniques.
Download and read online Developmental Biology of GH Secretion Growth and Treatment in PDF and EPUB Comprehensive update on developmental aspects of growth In the last few years, rapid progress has taken place in our understanding of the developmental biology of GH secretion and the pivotal role it plays in growth. This book keeps the reader updated on the most important developmental aspects and influences leading to changes in terms of clinical views. In ten chapters, well-known scientists and clinicians cover some of the most important progress made in recent times. The first chapters discuss pituitary gland development and imaging in detail followed by a comprehensive presentation of the genetics of the GH axis. Further chapters present a detailed overview of the epigenetics and bioinformatics of GH. This collection of up-to-date investigative data and reviews is of relevance not only to scientists involved in endocrinology but also to any physician interested in growth and development.
Download and read online The Zebrafish Cellular and Developmental Biology Part A Cellular Biology in PDF and EPUB The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology, is the latest edition in the Methods in Cell Biology series that looks at methods for analyzing cellular and developmental biology of zebrafish. Chapters cover such topics as cell biology and developmental and neural biology. Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies Written by experts in the field Contains cutting-edge material on the topic of developmental biology in zebrafish New two part edition of this important volume
Download and read online Developmental Biology of the Sea Urchin and Other Marine Invertebrates in PDF and EPUB In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory. These include methods and protocols on imaging, other useful experimental tools for cell, developmental biology research, variety of molecular biological methods, and strategies for utilizing the sea urchin genome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Developmental Biology of the Sea Urchin and other Marine Invertebrates: Methods and Protocols seeks to aid scientists in the further study into sea urchins and other marine invertebrates.
Download and read online Small Animal Imaging in PDF and EPUB Small animal imaging has been recognized as an important tool in preclinical research. Nevertheless, the results of non-invasive imaging are often disappointing owing to choice of a suboptimal imaging modality and/or shortcomings in study design, experimental setup, and data evaluation. This textbook is a practical guide to the use of non-invasive imaging in preclinical research. Each of the available imaging modalities is discussed in detail, with the assistance of numerous informative illustrations. In addition, many useful hints are provided on the installation of a small animal unit, study planning, animal handling, and the cost-effective performance of small animal imaging. Cross-calibration methods, data postprocessing, and special imaging applications are also considered in depth. This is the first book to cover all the practical basics in small animal imaging, and it will prove an invaluable aid for researchers, students, and technicians.
Download and read online Imaging in Neuroscience in PDF and EPUB As imaging technologies have revolutionized research in many areas of biology and medicine, neuroscientists have often pioneered the use of these new visualization techniques. This volume is an essential guide to discovering and implementing these techniques in the neuroscience lab.
Download and read online Reproductive and Developmental Toxicology in PDF and EPUB Reproductive and Developmental Toxicology, Second Edition, is a comprehensive and authoritative resource that provides the latest literature on this complex subject with a primary focus on three core components—parent, placenta, and fetus—and the continuous changes that occur in each. Enriched with relevant references describing every aspect of reproductive toxicology, this revised and updated resource addresses the totality of the subject, discussing a broad range of topics, including nanoparticles and radiation, gases and solvents, smoking, alcohol and drug abuse, and metals, amongst others. With a special focus on placental toxicity, this book is the only available reference to connect the three key risk stages, also including discussions on reproductive and developmental toxicity in domestic animals, fish, and wildlife. Completely revised and updated to include the most recent developments in the field, the book is an essential resource for advanced students and researchers in toxicology, as well as biologists, pharmacologists, and teratologists from academia, industry, and regulatory agencies. Provides a complete, up-to-date, integrated source of information on the key risk stages during reproduction and development Includes new chapters covering significant developments, such as dose-response assessment for developmental toxicity, juvenile toxicity, and neural tube defects, as well as emerging science, such as stem cell application, toxicoproteomics, metabolomics, endocrine disruption, surveillance and regulatory considerations, and risk assessment Offers diverse and unique in vitro and in vivo toxicity models for reproductive and developmental toxicity testing in a user-friendly format that assists in comparative analysis
Download and read online The Zebrafish Cellular and Developmental Biology in PDF and EPUB This volume of Methods in Cell Biology, the first of 3 parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases. Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls Summarizes the Zebrafish Genome Project
Download and read online Laboratory Methods in Cell Biology Imaging in PDF and EPUB Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields
Download and read online Live Imaging in Zebrafish in PDF and EPUB The zebrafish embryo has now been well established as an organism of choice for the genetic and cell biological basis of vertebrate development. Large-scale forward genetic screens together with reverse genetic tools have resulted in a flood of new information on the regulation of vertebrate development. Furthermore, the unique transparency of the zebrafish embryo allows unprecedented access to cells and tissues and many important discoveries on gene function have already been made using live imaging techniques in this organism. This book successfully brings together state-of-the-art methodologies of live imaging, such as the use of fluorescence correlation spectroscopy (FCS), that will be useful for investigators in the field of developmental biology to address their favorite problem using cell biological approaches in the zebrafish One of the key reasons that has made the zebrafish such a popular vertebrate model is its transparency and its potential for high-resolution cell biology and imaging. However, there is no comprehensive and up-to-date guide to the new "live imaging" tools that can now be used in the zebrafish. Furthermore, there are many new microscopy methods that have been adapted for use in zebrafish, and this book can be a good guide even for scientists already familiar with the imaging advantages of this model.
Download and read online Imaging in Neuroscience and Development in PDF and EPUB As imaging studies have continued to expand in scope and sophistication, this new edition of the highly successful and well–received Imaging Neurons: A Laboratory Manualhas expanded to include development, with over twenty new chapters on such topics as MRI microscopy, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes over forty sections of protocols for imaging techniques.
Download and read online Quantitative Imaging in Cell Biology in PDF and EPUB This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Download and read online Craniofacial Development in PDF and EPUB Craniofacial Development, the latest volume of Current Topics in Developmental Biology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in Craniofacial Development, and includes sections on such topics as microRNAs in craniofacial development and epigenetic regulation in craniofacial development. Provides a comprehensive book on craniofacial development and tissue regeneration Authored by leading experts in this field Carefully organized to cover an array of topics critical in helping readers learn the most important aspects of craniofacial development and tissue regeneration
Download and read online Magnetic Resonance Imaging in PDF and EPUB Magnetic Resonance Imaging: Methods and Biologic Applications discusses the progress that has been made in the area of MRI, and its potential to offer much more in terms of understanding basic biology and physiology both in animals and humans. Topics covered in this text include: MRI of small and large animal physiology, temperature/pH/pO2 measurements, and MRS of the brain and heart.
Download and read online Optical Coherence Tomography in PDF and EPUB Optical coherence tomography (OCT) is the optical analog of ultrasound imaging and is emerging as a powerful imaging technique that enables non-invasive, in vivo, high resolution, cross-sectional imaging in biological tissue. This book introduces OCT technology and applications not only from an optical and technological viewpoint, but also from biomedical and clinical perspectives. The chapters are written by leading research groups, in a style comprehensible to a broad audience.