Download and read online Crispr Cas A Laboratory Manual in PDF and EPUB The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign DNA, these powerful techniques allow investigators to edit DNA sequences and modulate gene expression more rapidly and accurately than ever before. Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system-especially for minimizing off-target effects-are also provided. Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.
Download and read online Introduction to Experimental Biophysics Second Edition in PDF and EPUB Praise for the First Edition “essential reading for any physical scientist who is interested in performing biological research.” ?Contemporary Physics "an ambitious text.... Each chapter contains protocols and the conceptual reasoning behind them, which is often useful to physicists performing biological experiments for the first time." –Physics Today This fully updated and expanded text is the best starting point for any student or researcher in the physical sciences to gain firm grounding in the techniques employed in molecular biophysics and quantitative biology. It includes brand new chapters on gene expression techniques, advanced techniques in biological light microscopy (super-resolution, two-photon, and fluorescence lifetime imaging), holography, and gold nanoparticles used in medicine. The author shares invaluable practical tips and insider’s knowledge to simplify potentially confusing techniques. The reader is guided through easy-to-follow examples carried out from start to finish with practical tips and insider’s knowledge. The emphasis is on building comfort with getting hands "wet" with basic methods and finally understanding when and how to apply or adapt them to address different questions. Jay L. Nadeau is a scientific researcher and head of the Biomedical Engineering in Advanced Applications of Quantum, Oscillatory, and Nanotechnological Systems (BEAAQONS) lab at Caltech and was previously associate professor of biomedical engineering and physics at McGill University.
Download and read online Wirtschaftliches Grundwissen in PDF and EPUB Wenn frisch gebackene Absolventen eines natur- oder ingenieurwissenschaftlichen Studienganges in ihr erstes Wirtschaftsunternehmen kommen, landen sie auf einem fremden Planeten: dem der Kaufleute, Betriebswirte, Juristen und Manager. Um dort bestehen zu k?nnen, ist zumindest ein Grundwissen dar?ber, wie Wirtschaft funktioniert und geregelt ist, unverzichtbar. Dieser Kompaktkurs f?r das moderne Wirtschaftsleben spart nichts aus: Von der doppelten Buchf?hrung bis zur Erschlie?ung globaler M?rkte - hier wird alles in leicht verst?ndlichen Begriffen erkl?rt. - Der Crash-Kurs f?r Berufsanf?nger: Nimmt die Scheu vor dem Umgang mit Geld, M?rkten und Produkten. Alle Aspekte des Wirtschaftslebens werden dargestellt, ohne unn?tigen Ballast und ohne "Fachchinesisch". - Praxisnah: Zahlreiche Fallbeispiele und ?bungsaufgaben zu aktuellen Themen aus dem Wirtschaftleben - mit L?sungen im Anhang! Von der Markteinf?hrung eines 3-Liter-Autos bis zur Existenzgr?ndung als "Ich-AG" werden reale Szenarien erkl?rt und analysiert. - Lesefreundliches Layout: Eine Doppelseite zu jedem Thema, mit klar gekennzeichneten Zusammenfassungen und Beispielen. - Aktuell: Alle Kapitel ber?cksichtigen jetzt die ?nderungen in der nationalen und internationalen Gesetzgebung bis 2006 (z. B. neue Rechtsformen bei Gesellschaften, neue Regelungen beim K?ndigungsschutz, Ich-AGs, etc.) - alle ?nderungen in den wirtschaftlichen und politischen Rahmenbedingungen der letzten 5 Jahre sind ber?cksichtigt.
Download and read online Molecular and Biochemical Toxicology in PDF and EPUB Written as an advanced text for toxicology students, this book is much more than an introduction and provides in-depth information describing the underlying mechanisms through which toxicants produce their adverse responses. • Links traditional toxicology to modern molecular techniques, important for teaching to graduate courses and professional studies • Uses a didactic approach with basic biological or theoretical background for the methodology presented • Brings together and comprehensively covers a range of dynamic aspects in biochemical and molecular toxicology • Guides student and professional toxicologists in comprehending a broad range of issues, compiled and authored by a diverse group of experts • “A good introductory textbook covering the biochemical toxicology of organic substances and the relevant methodology in some detail.... It offers good value for money and can be recommended as a textbook for appropriate courses” – BTS Newsletter review of the 4th edition
Download and read online Taschenatlas der Biotechnologie und Gentechnik in PDF and EPUB Der Taschenatlas der Biotechnologie und Gentechnik hat seit dem ersten Erscheinen 2001 bereits etliche Jahrgänge von Studenten und Schülern und interessierte Quereinsteiger in die Grundlagen diese wegweisenden Zukunftstechnologien eingeführt und sich als anschaulicher und unersetzlicher Begleiter etabliert. Biotechnologie und Gentechnik gelten als Schlüsseltechnologien des 21. Jahrhunderts. Sie sind Motor für die Erforschung und Entwicklung neuer Arzneimittel und Therapieformen, von Nutzpflanzen und Lebensmittel sowie von modernen Umwelttechnologien und innovativen industriellen Verfahren. Diese neue Auflage wurde grundlegend aktualisiert sowie um die Themen Tissue Engineering, Protein Design und Proteomics erweitert. Der neue Atlas wird damit weiterhin seiner Rolle als reichhaltige und aktuelle Quelle zu den spannendsten Themen innerhalb dieses wichtigen Forschungszweiges gerecht. aus einer Rezension der 1. Auflage: "... Der Atlas ist Studenten der Naturwissenschaften und der Medizin ebenso zu empfehlen wie allen, die einen Überblick über Produkte, Methoden, Anwendungen sowie wirtschaftliche und rechtliche Rahmenbedingungen der Bio- und Gentechnologie suchen." Chemie in unserer Zeit
Download and read online Laboratory Animal Medicine in PDF and EPUB Laboratory Animal Medicine, Third Edition, is a fully revised publication from the American College of Laboratory Medicine’s acclaimed blue book series. It presents an up-to-date volume that offers the most thorough coverage of the biology, health, and care of laboratory animals. The book is organized by species, with new inclusions of chinchillas, birds, and program and employee management, and is written and edited by known experts in the fields. Users will find gold-standard guidance on the study of laboratory animal science, as well as valuable information that applies across all of the biological and biomedical sciences that work with animals. Organized by species for in-depth understanding of biology, health, and best care of animals Features the inclusion of chinchillas, quail, and zebra finches as animal models Offers guidance on program and employee management Covers regulations, policies, and laws for laboratory animal management worldwide
Download and read online Genome Editing in PDF and EPUB This timely volume explores the use of CRISPR-Cas9 for genome editing, presenting cutting-edge techniques and their applications in treatment of disease. The chapters describe latest methods such as use of targetable nucleases, investigation of the non-coding genome, mouse genome editing, increasing of knock-in efficiency in mouse zygotes, and generation of reporter stem cells; the text contextualizes these methods in treatment of cardiovascular disease, diabetes mellitus, retinitis pigmentosa, and others. The final chapters round out the book with a discussion of controversies and future directions. Genome Editing is an essential, of-the-moment contribution to this rapidly growing field. Drawing from a wealth of international perspectives, it presents novel techniques and applications for the engineering of the human genome. This book is essential reading for all clinicians and researchers in stem cells, regenerative medicine, genomics, biochemical and biomedical engineering- especially those interested in learning more about genome editing and applying it in a targeted, specific way.
Download and read online Congenital Heart Diseases The Broken Heart in PDF and EPUB This book provides comprehensive insights into congenital heart disease from embryonic development through to clinical features, including human genetics and our current knowledge of the underlying molecular pathways. It is divided into three parts: an introduction to the development of the heart and its vessels, an overview of the molecular pathways affecting the development of various cardiovascular structures, and a main section focusing on the different types of structural and nonstructural congenital heart diseases, including their clinical features, underlying genetic alterations and related animal models and pathways. Taken together these chapters, which were written by and for clinicians and researchers, provide an integrated and cutting-edge resource for all those who want to learn more about both the clinical aspects and the genetic and molecular basis of congenital heart disease.
Download and read online Principles of Development in PDF and EPUB The process of biological development is an amazing feat of tightly regulated cellular behaviours--differentiation, movement, and growth--powerful enough to result in the emergence of a highly complex living organism from a single cell: the fertilized egg. Principles of Development clearlyillustrates the universal principles that govern this process of development in a succinct and accessible style. Cutting-edge science is explained clearly and succinctly, richly illustrated with a variety of custom drawn figures, animations, and online resources. A focus on the key principles of development throughout the text provides a framework on which a richer understanding of specific topics can bebuilt.
Download and read online Harpers Illustrated Biochemistry 30th Edition in PDF and EPUB Gain a thorough understanding of the principles of biochemistry as they relate to the study of clinical medicine THE BEST REVIEW FOR THE USMLE! The Thirtieth Edition of Harper’s Illustrated Biochemistry combines outstanding full-color illustrations with authoritative integrated coverage of biochemical disease and clinical information. Using brevity and numerous medically relevant examples, Harper's presents a clear, succinct review of the fundamentals of biochemistry that every student must understand in order to succeed in medical school. All fifty-eight chapters emphasize the medical relevance of biochemistry Full-color presentation includes more than 600 illustrations Each chapter includes a section on Biomedical Importance and a summary of the topics covered Review questions follow each of the eleven sections Case studies in every chapter emphasize the clinical relevance to biochemistry NEW coverage of toxic naturally-occurring amino acids; extraterrestrial biomolecules; computer-aided drug design; the role of complement cascade in bacterial and viral infection; secreted mediators of cell-cell signaling between leukocytes; the role of mast cells, basophils, and eosinophils; and the hazard of antioxidants that down-regulate radical signaling for apoptosis and increase risk of cancer Applauded by medical students for its current and engaging style, Harper's Illustrated Biochemistry is an essential for USMLE review and the single best reference for learning the clinical relevance of any biochemistry topic.
Download and read online Biologie der Pflanzen in PDF and EPUB Biology of Plants provides a comprehensive survey of basic botany including viruses, prokaryotes, fungi and protists. The 4th edition incorporates the newest scientific advances on all fronts, including increased emphasis on molecular methods applied to the study of plants, fundamentally new understanding of the evolution of angiosperms, substantial changes in the classification of protists and seedless vascular plants, significant new information on plant hormones from "Arabidopsis" studies. Conceived for the Americanundergraduate program, Raven offers an effective and goal-oriented exam preparation for both majors and minors in Botany (Diplom, Bachelor and Masters programs). "
Download and read online Oversexed and underfucked in PDF and EPUB Oversexed and underfucked! Diese sicherlich pointiert formulierte und auch provozierende Diagnose über den gegenwärtigen Zustand des ‚sexuellen Begehrens’ unterstellt, dass die Lust der Geschlechter aufeinander heute weitgehend abhanden gekommen sei und dass die vermeintliche, ganz wesentlich durch die Medien getragene, Übersexualisierung daran einen erheblichen Anteil habe. Und sie impliziert, dass ‚wir’ alle wissen (wollen), was guter Sex ist. Entlang dieser Leitnahmen geht die vorliegende Arbeit der gesellschaftlichen Konstruktion der Lust auf den Grund. Sie analysiert die Ideen einer ‚gelingenden Sexualität’ aus der Sicht der Wissenschaft ebenso wie das Rezeptwissen erfolgreicher Sexualratgeber von 1950 bis heute. Die aus diesen Quellen ‚rekonstruierten’ sexuellen Skripte (Dornröschen-Skript, Ken&Barbie-Skript, Adam&Eva-Skript und Ich&Ich-Skript) weisen – insgesamt betrachtet – eine typische Entwicklungslinie auf, die von der Darstellung des Geschlechtsverkehrs als Drama im Dornröschen-Skript bis hin zur als Porncast inszenierten Selbstbefriedigung im Ich&Ich-Skript führt. Der Begriff ‚underfucked’ zeigt somit nicht einen Mangel an Geschlechtsverkehr an, sondern beschreibt vielmehr seinen fortschreitenden ‚sozialen Tod’.
Download and read online International Review of Cell and Molecular Biology in PDF and EPUB International Review of Cell and Molecular Biology presents comprehensive reviews and current advances in cell and molecular biology, with articles addressing structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. The series has a worldwide readership, maintaining a high standard by publishing invited articles on important and timely topics authored by prominent cell and molecular biologists. Authored by some of the foremost scientists in the field Provides comprehensive reviews and current advances Brings a fresh perspective to those conducting research in cell biology, molecular biology, biochemistry, biotechnology, plant biology, physiology, and microbiology, among others Includes a wide range of perspectives on specific subjects Valuable reference material for advanced undergraduates, graduate students, and professional scientists
Download and read online Genetics of the Mouse in PDF and EPUB This book, written by experienced geneticists, covers topics ranging from the natural history of the mouse species, its handling and reproduction in the laboratory, and its classical genetics and cytogenetics, to modern issues including the analysis of the transcriptome, the parental imprinting and X-chromosome inactivation. The strategies for creating all sorts of mutations, either by genetic engineering or by using mutagens, are also reviewed and discussed in detail. Finally, a last chapter outlines the methodology used for the analysis of complex or quantitative traits. The authors also discuss the importance of accurate phenotyping, which is now performed in the mouse clinics established worldwide and identify the limits of the mouse model, which under certain circumstances can fail to present the phenotype expected from the cognate condition in the human model. For each chapter an up-to-date list of pertinent references is provided. In short, this book offers an essential resource for all scientists who use or plan to use mice in their research.
Download and read online Phage Therapy Past Present and Future in PDF and EPUB Historically, the first observation of a transmissible lytic agent that is specifically active against a bacterium (Bacillus anthracis) was by a Russian microbiologist Nikolay Gamaleya in 1898. At that time, however, it was too early to make a connection to another discovery made by Dmitri Ivanovsky in 1892 and Martinus Beijerinck in 1898 on a non-bacterial pathogen infecting tobacco plants. Thus the viral world was discovered in two of the three domains of life, and our current understanding is that viruses represent the most abundant biological entities on the planet. The potential of bacteriophages for infection treatment have been recognized after the discoveries by Frederick Twort and Felix d’Hérelle in 1915 and 1917. Subsequent phage therapy developments, however, have been overshadowed by the remarkable success of antibiotics in infection control and treatment, and phage therapy research and development persisted mostly in the former Soviet Union countries, Russia and Georgia, as well as in France and Poland. The dramatic rise of antibiotic resistance and especially of multi-drug resistance among human and animal bacterial pathogens, however, challenged the position of antibiotics as a single most important pillar for infection control and treatment. Thus there is a renewed interest in phage therapy as a possible additive/alternative therapy, especially for the infections that resist routine antibiotic treatment. The basis for the revival of phage therapy is affected by a number of issues that need to be resolved before it can enter the arena, which is traditionally reserved for antibiotics. Probably the most important is the regulatory issue: How should phage therapy be regulated? Similarly to drugs? Then the co-evolving nature of phage-bacterial host relationship will be a major hurdle for the production of consistent phage formulae. Or should we resort to the phage products such as lysins and the corresponding engineered versions in order to have accurate and consistent delivery doses? We still have very limited knowledge about the pharmacodynamics of phage therapy. More data, obtained in animal models, are necessary to evaluate the phage therapy efficiency compared, for example, to antibiotics. Another aspect is the safety of phage therapy. How do phages interact with the immune system and to what costs, or benefits? What are the risks, in the course of phage therapy, of transduction of undesirable properties such as virulence or antibiotic resistance genes? How frequent is the development of bacterial host resistance during phage therapy? Understanding these and many other aspects of phage therapy, basic and applied, is the main subject of this Topic.